6U00
Crystal Structure of Fungal RNA Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 55.436, 122.873, 150.618 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.907 - 1.981 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.23580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u32 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
Rmerge | 0.055 | 0.029 | 0.618 |
Rmeas | 0.060 | 0.032 | 0.676 |
Rpim | 0.024 | 0.013 | 0.269 |
Total number of observations | 211528 | ||
Number of reflections | 34731 | 1951 | 1281 |
<I/σ(I)> | 10.5 | ||
Completeness [%] | 95.8 | 99.1 | 73.7 |
Redundancy | 6.1 | 5.8 | 5.4 |
CC(1/2) | 0.999 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.3M NaH2PO4, 15%PEG3350 |