6TZV
Crystal Structure of the carboxyltransferase subunit of ACC (AccD6) in complex with inhibitor Phenyl-Cyclodiaone from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2016-02-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0,987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.345, 153.069, 150.184 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 35.760 - 2.388 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.760 | 2.480 |
| High resolution limit [Å] | 2.388 | 2.430 |
| Rmeas | 0.067 | |
| Rpim | 0.029 | |
| Number of reflections | 180446 | 2463 |
| <I/σ(I)> | 32.5 | |
| Completeness [%] | 98.7 | 98.2 |
| Redundancy | 1.36 | |
| CC(1/2) | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M Li2SO4, 0.1 M Tris pH 7, 1 M potassium sodium tartarate |
