6TZB

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin in complex with 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	210.401, 132.173, 72.468
Unit cell angles	90.00, 97.93, 90.00

Refinement procedure

Resolution	39.748 - 2.243
R-factor	0.1597
Rwork	0.157
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fnk
RMSD bond length	0.008
RMSD bond angle	0.969
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.243	4.680	2.250
Rmerge	0.099	0.039	0.895
Rmeas	0.108	0.043	0.981
Rpim	0.042	0.017	0.397
Number of reflections	93371	10524	10355
<I/σ(I)>	6.3
Completeness [%]	100.0	100	100
Redundancy	6.5	6.3	6
CC(1/2)		0.985	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M sodium cacodylate pH 6.5, 6% PEG 8000, and 39% 2-methyl-2,4-pentanediol