6TYW

Structure of Ku80 von Willebrand domain S229A mutant complexed with APLF Ku Binding Motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-07
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	43.847, 71.383, 79.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.806 - 1.700
R-factor	0.181250894086
Rwork	0.179
R-free	0.21816
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tyz
RMSD bond length	0.009
RMSD bond angle	0.914
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.699	1.700
Rmeas	0.057	0.156
Number of reflections	28054	1191
<I/σ(I)>	50.6
Completeness [%]	98.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20 % (w/v) PEG3350