6TYV

Structure of Ku80 von Willebrand domain complexed with WRN Ku Binding Motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-01
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	44.112, 71.351, 79.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.520 - 1.926
R-factor	0.194656196721
Rwork	0.193
R-free	0.23484
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jeq
RMSD bond length	0.008
RMSD bond angle	0.865
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Rmeas	0.070	0.496
Number of reflections	18919	914
<I/σ(I)>	34.3
Completeness [%]	99.5
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Na-HEPES pH 7.5, 25 % (w/v) PEG3000