6TYU

Structure of Ku80 von Willebrand domain complexed with MRI Ku Binding Motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-01
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	44.574, 71.350, 79.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.803 - 1.469
R-factor	0.179161282252
Rwork	0.178
R-free	0.20077
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tyv
RMSD bond length	0.008
RMSD bond angle	0.905
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.468	1.470
Rmeas	0.048	0.602
Number of reflections	43410	1900
<I/σ(I)>	53
Completeness [%]	99.1
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Potassium formate, 20 % (w/v) PEG3350