6TY1
Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228Ser) from an H10N7 seal influenza virus isolated in Germany in complex with human receptor analogue 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.990, 212.670, 154.900 |
| Unit cell angles | 90.00, 101.58, 90.00 |
Refinement procedure
| Resolution | 87.080 - 3.200 |
| R-factor | 0.2747 |
| Rwork | 0.272 |
| R-free | 0.33640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.945 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.080 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 71930 | 5351 |
| <I/σ(I)> | 6.6 | 1.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.997 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 6% PEG3350, 0.1 M MES pH6.5 |
