6TY0
NT PART CRYSTAL STRUCTURE OF THE RYMV-ENCODED VIRAL RNA SILENCING SUPPRESSOR P1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 195.612, 195.612, 195.612 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 112.940 - 2.100 |
R-factor | 0.18564 |
Rwork | 0.184 |
R-free | 0.21417 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.709 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | HKL2Map |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 112.940 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.880 |
Rmeas | 0.058 | |
Rpim | 0.012 | |
Number of reflections | 22243 | 3160 |
<I/σ(I)> | 45 | 6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 293 | 20 mM Tris pH 8, 75 mM NaCl, 1 mM DTT and 300 mM ZnCl2 |