6TXK

Crystal structure of thermotoga maritima E65K Ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.3
Synchrotron site	BESSY
Beamline	14.3
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-18
Detector	RAYONIX MX-225
Wavelength(s)	0.895
Spacegroup name	H 3 2
Unit cell lengths	175.811, 175.811, 354.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.440 - 2.359
R-factor	0.2019
Rwork	0.201
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.440	20.440	2.500
High resolution limit [Å]	2.359	7.010	2.360
Rmerge	0.230	0.050	1.531
Rmeas	0.248	0.055	1.645
Number of reflections	86438	3414	13809
<I/σ(I)>	8.72	28.04	1.33
Completeness [%]	99.8	97.5	99.6
Redundancy	7.516	6.145	7.648
CC(1/2)	0.992	0.998	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2.4 M (NH4)2 SO4, 0.1M MES. pH 6.0