6TXJ
Crystal structure of thermotoga maritima A42V E65D Ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-13 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 176.203, 176.203, 352.794 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.863 - 2.170 |
| R-factor | 0.2105 |
| Rwork | 0.210 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vlg |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.15.1-3469) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 24.863 | 24.863 | 2.300 |
| High resolution limit [Å] | 2.167 | 6.450 | 2.170 |
| Rmerge | 0.129 | 0.028 | 4.047 |
| Rmeas | 0.136 | 0.029 | 4.258 |
| Number of reflections | 111200 | 4455 | 17634 |
| <I/σ(I)> | 12.99 | 64.94 | 0.53 |
| Completeness [%] | 99.7 | 99.5 | 98.8 |
| Redundancy | 10.147 | 9.34 | 10.306 |
| CC(1/2) | 0.999 | 1.000 | 0.324 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.4 M (NH4)2 SO4, 0.1M MES. pH 6.0 |
