6TWH
Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228Ser) from an H10N7 seal influenza virus isolated in Germany
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.310, 213.610, 156.800 |
Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
Resolution | 72.110 - 2.680 |
R-factor | 0.2706 |
Rwork | 0.269 |
R-free | 0.30170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d00 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.818 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.110 | 2.750 |
High resolution limit [Å] | 2.680 | 2.680 |
Number of reflections | 124460 | 9164 |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.9 | |
Redundancy | 7 | |
CC(1/2) | 0.998 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 6% PEG3350, 0.1 M MES pH6.5 |