6TVS
Crystal structure of the haemagglutinin mutant (Gln226Leu) from an H10N7 seal influenza virus isolated in Germany in complex with avian receptor analogue 3'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.340, 218.360, 73.060 |
| Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
| Resolution | 51.540 - 1.900 |
| R-factor | 0.2308 |
| Rwork | 0.230 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.078 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.280 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 164307 | 8151 |
| <I/σ(I)> | 11.3 | 1.3 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.998 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 4% PEG3350, 0.1M HEPES pH 7.5 |
