6TVR
Crystal structure of the haemagglutinin mutant (Gln226Leu) from an H10N7 seal influenza virus isolated in Germany
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.354, 214.102, 157.165 |
| Unit cell angles | 90.00, 102.06, 90.00 |
Refinement procedure
| Resolution | 72.330 - 2.630 |
| R-factor | 0.2419 |
| Rwork | 0.241 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.785 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.330 | 2.670 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 132596 | 6585 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.981 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 4% PEG3350, 0.1M HEPES pH 7.5 |
