6TVG
Human CD73 (ecto 5'-nucleotidase) in complex with AMPCP in the open state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-04 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.89429 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.259, 131.268, 66.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.190 - 1.480 |
R-factor | 0.1298 |
Rwork | 0.128 |
R-free | 0.16740 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4h2g |
RMSD bond length | 0.007 |
RMSD bond angle | 0.986 |
Data reduction software | XDS (2016) |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.190 | 1.530 |
High resolution limit [Å] | 1.477 | 1.477 |
Rmeas | 0.116 | 0.993 |
Number of reflections | 98145 | 4759 |
<I/σ(I)> | 12 | 1.7 |
Completeness [%] | 99.4 | |
Redundancy | 4.96 | |
CC(1/2) | 0.997 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Tris pH 7.8, 10 % PEG6000, 30 mM AMPCP, 10 % DMSO, 10 % glycerol |