6TVD
Crystal structure of the haemagglutinin from a H10N7 seal influenza virus isolated in Germany in complex with avian receptor analogue, 3'-SLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.741, 214.956, 157.781 |
Unit cell angles | 90.00, 100.60, 90.00 |
Refinement procedure
Resolution | 68.550 - 2.700 |
R-factor | 0.2462 |
Rwork | 0.243 |
R-free | 0.31260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d00 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.841 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.550 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 123642 | 6123 |
<I/σ(I)> | 10.4 | |
Completeness [%] | 99.1 | |
Redundancy | 3 | |
CC(1/2) | 0.994 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2%-4% PEG6000, 0.1M HEPES pH7.0 |