6TV7
Crystal structure of rsGCaMP in the OFF state (illuminated)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-28 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.999995 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 120.670, 120.670, 96.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.090 - 2.900 |
R-factor | 0.1855 |
Rwork | 0.182 |
R-free | 0.25040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ek4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.897 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 16254 | 1174 |
<I/σ(I)> | 11.17 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 13 | |
CC(1/2) | 0.996 | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.21 M sodium formate, 0.1 M Bis-Tris-Propane buffer pH 8.5, 18% (w/v) PEG 3350 |