6TUV

Crystal structure of Mindy1 in complex with Lys48 linked di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.939274
Spacegroup name	P 41 2 2
Unit cell lengths	73.862, 73.862, 202.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.370 - 2.160
R-factor	0.2096
Rwork	0.207
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jkn 1ubq
RMSD bond length	0.009
RMSD bond angle	1.608
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.810	2.230
High resolution limit [Å]	2.160	2.160
Rmerge	0.087	0.955
Rmeas	0.094	1.028
Rpim	0.036	0.378
Total number of observations	212300
Number of reflections	31102	2629
<I/σ(I)>	13.9	4.1
Completeness [%]	99.9	99.9
Redundancy	6.8	7.3
CC(1/2)	0.999	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	100 mM Tris-HCl, 200mM LiSO4.H2O, 30% PEG400