6TUS

human XPG, Apo2 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9787
Spacegroup name	P 42 21 2
Unit cell lengths	134.225, 134.225, 110.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.850 - 2.500
R-factor	0.2038
Rwork	0.203
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tur
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.850	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	35554	3887
<I/σ(I)>	14.4
Completeness [%]	99.8
Redundancy	7.2
CC(1/2)	0.999	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	25% PEG 3350, 100 mM Bis-Tris pH 6.5, 200 mM Amm. Sulfate.