6TTV
Crystal structure of the human METTL3-METTL14 complex bound to Compound 3 (ASI_M3M_138)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-24 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.150, 64.150, 225.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.670 - 2.140 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l6d |
RMSD bond length | 0.008 |
RMSD bond angle | 0.933 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.270 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 30741 | 4796 |
<I/σ(I)> | 14.53 | |
Completeness [%] | 99.9 | |
Redundancy | 8.25 | |
CC(1/2) | 0.999 | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 200 mM Magnesium acetate |