6TSZ
The ULK4 Pseudokinase Domain Bound To ATPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.442, 41.468, 69.755 |
| Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
| Resolution | 36.320 - 1.900 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fdy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.846 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.470 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 25024 | 1609 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 95.6 | 97.4 |
| Redundancy | 4.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M citrate pH 5.9, 15% 2 propanol, 6% PEG4K |
