6TRU

Crystal structure of the N-terminal half of the TFIIH subunit p52

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97973
Spacegroup name	P 31
Unit cell lengths	104.309, 104.309, 164.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.970 - 2.800
R-factor	0.1875
Rwork	0.186
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.438
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.970	2.890
High resolution limit [Å]	2.800	2.800
Rmerge	0.162	2.829
Rpim	0.053	0.901
Number of reflections	49518	4628
<I/σ(I)>	10.7	1
Completeness [%]	100.0	100
Redundancy	10.4	10.8
CC(1/2)	0.997	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293.15	calcium acetate, PEG 8000, HEPES