6TRQ

S.c. Scavenger Decapping Enzyme DcpS in complex with the capped RNA dinucleotide m7G-GU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.999970
Spacegroup name	P 21 21 21
Unit cell lengths	87.761, 104.103, 189.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.306 - 2.944
R-factor	0.2072
Rwork	0.204
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bv3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.306	47.306	3.020
High resolution limit [Å]	2.940	13.170	2.940
Rmerge	0.312	0.048	2.470
Rmeas	0.325	0.050	2.565
Number of reflections	70791	493	2651
<I/σ(I)>	9.11	36.92	1.19
Completeness [%]	99.7	96.9	96.6
Redundancy	13.338	11.714	13.635
CC(1/2)	0.995	0.999	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM NaCl 100 mM Hepes pH 7.5 1.6 M NH4SO4