6TRL

Human Eg5 motor domain mutant Y82F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-12
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.966000
Spacegroup name	P 1 21 1
Unit cell lengths	53.075, 77.430, 91.076
Unit cell angles	90.00, 91.81, 90.00

Refinement procedure

Resolution	39.850 - 2.200
R-factor	0.1955
Rwork	0.195
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ii6
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.480	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.034	0.446
Rmeas	0.044	0.583
Rpim	0.028	0.371
Number of reflections	56409	1934
<I/σ(I)>	12.8	2
Completeness [%]	88.0	59.8
Redundancy	2.2	2.1
CC(1/2)	0.999	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277.15	PEG3350, NaNO3, MES