6TR3
Ruminococcus gnavus GH29 fucosidase E1_10125 in complex with fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.219, 48.643, 80.287 |
| Unit cell angles | 90.00, 115.02, 90.00 |
Refinement procedure
| Resolution | 63.940 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oue |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.215 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.940 | 63.900 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmeas | 0.096 | 0.049 | 0.716 |
| Rpim | 0.054 | 0.027 | 0.409 |
| Total number of observations | 8882 | 7972 | |
| Number of reflections | 55407 | 2919 | 2730 |
| <I/σ(I)> | 6.9 | 18.1 | 1.1 |
| Completeness [%] | 99.1 | 99.6 | 97.5 |
| Redundancy | 3.1 | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M magnesium chloride, 25% PEG 3350, 0.1 M bis-tris pH 5.5 20mg/ml protein |
