6TQR

The crystal structure of the MSP domain of human VAP-A in complex with the Phospho-FFAT motif of STARD3.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	39.140, 43.780, 83.640
Unit cell angles	89.46, 92.91, 105.13

Refinement procedure

Resolution	42.260 - 1.850
R-factor	0.2068
Rwork	0.204
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z9o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev-3699)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.260	42.260	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.208	0.043
Rmeas	0.248	0.051
Rpim	0.133
Number of reflections	42485	512	1936
<I/σ(I)>	4.57	25.58
Completeness [%]	92.8	100	57.1
Redundancy	3.338	3.49	2.456
CC(1/2)	0.990	0.997	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% PEG 2000 MME, 0.1 M HEPES pH 7.5