6TQJ
Crystal structure of the c14 ring of the F1FO ATP synthase from spinach chloroplast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 93.136, 96.336, 158.684 |
Unit cell angles | 90.00, 106.72, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.2058 |
Rwork | 0.204 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v3c |
RMSD bond length | 0.002 |
RMSD bond angle | 1.068 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.170 | 48.170 | 2.360 |
High resolution limit [Å] | 2.300 | 10.030 | 2.300 |
Rmerge | 0.192 | 0.051 | 1.169 |
Rmeas | 0.225 | 0.060 | 1.369 |
Rpim | 0.114 | 0.031 | 0.704 |
Total number of observations | 214591 | 2592 | 12342 |
Number of reflections | 57691 | 753 | 3484 |
<I/σ(I)> | 4.9 | 11.5 | 1.2 |
Completeness [%] | 96.7 | 98.2 | 75.6 |
Redundancy | 3.7 | 3.4 | 3.5 |
CC(1/2) | 0.993 | 0.998 | 0.421 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1 M MES pH 5.8, 1.4 M ammonium sulfate |