6TPI
EnvC bound to the FtsX periplasmic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.031, 100.470, 107.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.820 - 2.100 |
R-factor | 0.2177 |
Rwork | 0.215 |
R-free | 0.27470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4BH5 and 4N8O |
RMSD bond length | 0.007 |
RMSD bond angle | 1.476 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.823 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.044 | 0.577 |
Number of reflections | 43252 | 3494 |
<I/σ(I)> | 15 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12 | 10.8 |
CC(1/2) | 0.999 | 0.767 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 10% PEG 8000, 20 % Ethylene glycol, 0.1 M Tris/bicine pH8.5, ) 0.12M monosaccarides (glucose, mannose, galactose, fucose, xylose, NAG) |