6TOU

Rabies virus glycoprotein PH domain in complex with the scFv fragment of broadly neutralizing human antibody RVC20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	81.945, 81.945, 155.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.506 - 2.587
R-factor	0.1932
Rwork	0.190
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Phyre2 homology model of RVC20 scFv
RMSD bond length	0.003
RMSD bond angle	0.607
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.506	2.720
High resolution limit [Å]	2.587	2.587
Rmeas	0.106	3.758
Rpim	0.021	0.705
Number of reflections	15457	774
<I/σ(I)>	21.374
Completeness [%]	89.5	33.3
Redundancy	25.7	28.1
CC(1/2)	0.999	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.7 uL of 18 mg/mL complex in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.7 uL of reservoir solution containing 100 mM Tris-Cl pH 8.0, 300 mM CaCl2, 22% w/v PEG4000