6TOP

Structure of the PorE C-terminal domain, a protein of T9SS from Porphyromonas gingivalis

Replaces:  6I9O

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97911
Spacegroup name	C 1 2 1
Unit cell lengths	208.030, 52.220, 66.560
Unit cell angles	90.00, 108.38, 90.00

Refinement procedure

Resolution	49.000 - 1.550
R-factor	0.1911
Rwork	0.190
R-free	0.21520
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	1.590
High resolution limit [Å]	1.550	1.550
Rmeas	0.090
Rpim	0.019
Number of reflections	96340	5637
<I/σ(I)>	19.7
Completeness [%]	97.6	77.6
Redundancy	22	14
CC(1/2)	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M sodium acetate 0.1M MES 30% (w/v) PEG2000