6TO5

Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	70.400, 70.400, 148.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.170 - 2.380
R-factor	0.2153
Rwork	0.214
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iit
RMSD bond length	0.004
RMSD bond angle	0.600
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.520
High resolution limit [Å]	2.380	2.380
Rmeas	0.189	2.210
Number of reflections	16576	2262
<I/σ(I)>	14.9	1.48
Completeness [%]	99.8	98.8
Redundancy	20.8
CC(1/2)	1.000	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	298	0.1 M phosphate citrate pH 4.2, 0.2 M NaCl, 20% (w/v) PEG 8000