6TNQ

Crystal structure of the human Arc N-lobe bound to repeat 4 from GKAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	50.800, 29.370, 71.360
Unit cell angles	90.00, 93.40, 90.00

Refinement procedure

Resolution	100.000 - 1.300
Rwork	0.132
R-free	0.17040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x3h
RMSD bond length	0.016
RMSD bond angle	1.405
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.340
High resolution limit [Å]	1.300	1.300
Rmeas	0.032	0.179
Number of reflections	46481	1546
<I/σ(I)>	24.2	4.6
Completeness [%]	89.5	40.4
Redundancy	3.4	2
CC(1/2)	0.999	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	1.2 M sodium citrate