6TNH
Deoxyguanylosuccinate synthase (DgsS) quaternary structure with AMPPcP, dGMP, Asp, Magnesium at 2.21 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-12 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.979346 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.640, 91.640, 189.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.380 - 2.210 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6flf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.380 | 2.340 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 40942 | 6300 |
| <I/σ(I)> | 6.88 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.960 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1M LiSO4, 2%PEG 8000 |
