6TN7

Crystal structure of the human Arc C-lobe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 2 21 21
Unit cell lengths	37.210, 38.290, 61.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.670
Rwork	0.241
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x3x
RMSD bond length	0.004
RMSD bond angle	0.768
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.670	1.670
Rmeas	0.055	0.699
Number of reflections	9789	245
<I/σ(I)>	18.3	2.6
Completeness [%]	92.2
Redundancy	7.1	4.5
CC(1/2)	0.999	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	16% PEG 8000, 40 mM KH2PO4, 20% glycerol