6TMM
BIL2 domain from T.thermophila BUBL1 locus (C1A-N143A)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.477, 72.769, 70.264 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 69.602 - 2.398 |
| Rwork | 0.198 |
| R-free | 0.26650 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.602 | 2.540 |
| High resolution limit [Å] | 2.398 | 2.398 |
| Rmerge | 0.060 | 0.593 |
| Rmeas | 0.071 | 0.704 |
| Number of reflections | 26317 | 8236 |
| <I/σ(I)> | 12.25 | 1.78 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.36 | 3.37 |
| CC(1/2) | 0.998 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | Mg formate 0.1 M, PEG3350 19%, Tris 50 mM, NaCl 250 mM. |
