6TME

Monomeric LRX8 in complex with RALF4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000036
Spacegroup name	P 1
Unit cell lengths	51.806, 65.542, 83.700
Unit cell angles	72.12, 87.62, 68.36

Refinement procedure

Resolution	48.000 - 2.330
R-factor	0.1745
Rwork	0.172
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qxp
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.000	48.000	2.410
High resolution limit [Å]	2.330	9.010	2.330
Rmerge	0.136	0.058	0.908
Rmeas	0.161	0.068	1.073
Rpim	0.086	0.036	0.565
Total number of observations	142274	2502	12840
Number of reflections	40550	688	3700
<I/σ(I)>	6.6	19.6	1.4
Completeness [%]	97.2	95	90.4
Redundancy	3.5	3.6	3.5
CC(1/2)	0.991	0.993	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.3 M Ammonium sulfate 20 % w/v PEG 4000