6TM6

MUC2 CysD1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-10-03
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	54.879, 57.854, 27.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.230 - 1.630
R-factor	0.1774
Rwork	0.174
R-free	0.21120
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.961
Phasing software	SHELXDE
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.460	1.689
High resolution limit [Å]	1.630	1.630
Number of reflections	10882	737
<I/σ(I)>	37.79
Completeness [%]	94.9
Redundancy	22.9
CC(1/2)	1.000	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM citrate/phosphate buffer, pH 4.4, with 15% ethanol and 1% PEG 1K