6TLX

Crystal structure of the unconventional kinetochore protein Perkinsela sp. KKT2a central domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 64
Unit cell lengths	113.845, 113.845, 46.013
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.000 - 2.870
R-factor	0.2538
Rwork	0.253
R-free	0.27480
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	DIALS (1.8.4)
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.920	56.930	2.920
High resolution limit [Å]	2.870	7.770	2.870
Rmerge	0.102	0.048	1.045
Rmeas	0.105
Rpim	0.024		0.240
Number of reflections	7986	432	357
<I/σ(I)>	16.4	60.5	2.1
Completeness [%]	99.7	100	91.8
Redundancy	19.2	16.1	19.5
CC(1/2)	1.000	0.990	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	19% MPD, 50 mM Hepes, pH 7.5, 10 mM MgCl2