6TLE
Human Eg5 motor domain mutant E344K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-31 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.9666 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.930, 47.150, 85.500 |
| Unit cell angles | 93.91, 101.17, 109.23 |
Refinement procedure
| Resolution | 44.070 - 1.750 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ii6 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.070 | 1.777 |
| High resolution limit [Å] | 1.746 | 1.746 |
| Rmerge | 0.039 | 0.299 |
| Rmeas | 0.055 | 0.423 |
| Rpim | 0.039 | 0.299 |
| Number of reflections | 62425 | 3118 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 91.5 | 93 |
| Redundancy | 1.7 | 1.7 |
| CC(1/2) | 0.998 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277.15 | PEG3350, NaNO3 and MES pH 5.8 |
