6TLE

Human Eg5 motor domain mutant E344K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-31
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.9666
Spacegroup name	P 1
Unit cell lengths	46.930, 47.150, 85.500
Unit cell angles	93.91, 101.17, 109.23

Refinement procedure

Resolution	44.070 - 1.750
R-factor	0.2025
Rwork	0.201
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ii6
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.070	1.777
High resolution limit [Å]	1.746	1.746
Rmerge	0.039	0.299
Rmeas	0.055	0.423
Rpim	0.039	0.299
Number of reflections	62425	3118
<I/σ(I)>	11.2
Completeness [%]	91.5	93
Redundancy	1.7	1.7
CC(1/2)	0.998	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277.15	PEG3350, NaNO3 and MES pH 5.8