6TKY

Crystal structure of the DHR2 domain of DOCK10 in complex with CDC42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-09-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	96.898, 96.898, 310.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.830 - 2.550
R-factor	0.1913
Rwork	0.188
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wm9
RMSD bond length	0.008
RMSD bond angle	0.929
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.830	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	49296	5918
<I/σ(I)>	5.52
Completeness [%]	99.9
Redundancy	7.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	25% (w/v) PEG 3350, 200 mM potassium acetate, 8% (v/v) 1,1,1,3,3,3-Hexafluoro-2-propanol.