6TKB

ChiLob 7/4 H2 HC-C224S F(ab')2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.8
Spacegroup name	P 21 21 21
Unit cell lengths	74.758, 95.191, 150.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.641 - 2.000
Rwork	0.182
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fax
RMSD bond length	0.010
RMSD bond angle	1.615
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.641	2.040
High resolution limit [Å]	2.000	2.000
Rpim	0.018	0.342
Number of reflections	76835	4462
<I/σ(I)>	25.3	2.3
Completeness [%]	99.1
Redundancy	29.5
CC(1/2)	0.999	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 8000, cacodylate