6TJY
Crystal structure of haemagglutinin from (A/seal/Germany/1/2014) seal H10N7 influenza virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.421, 212.932, 156.901 |
| Unit cell angles | 90.00, 101.51, 90.00 |
Refinement procedure
| Resolution | 35.040 - 2.820 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.040 | 2.870 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Number of reflections | 105212 | 5226 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 98.2 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.995 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2%-4% PEG6000, 0.1M HEPES pH7.0 |
