6TJT

Neuropilin2-b1 domain in a complex with the C-terminal VEGFC peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	38.240, 76.544, 63.357
Unit cell angles	90.00, 96.06, 90.00

Refinement procedure

Resolution	48.640 - 1.310
R-factor	0.1545
Rwork	0.153
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rn5
RMSD bond length	0.008
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.000	63.000	1.380
High resolution limit [Å]	1.310	4.130	1.310
Number of reflections	86786	2758	12438
<I/σ(I)>	12.6
Completeness [%]	98.9
Redundancy	3.1
CC(1/2)	0.998	0.996	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M NaOAc, 0.1 M HEPES pH 7.5, 12 % (w/v) PEG4000