6TIU

DROSOPHILA GTP-TUBULIN Y222F MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	65.970, 126.350, 249.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 3.571
R-factor	0.222
Rwork	0.221
R-free	0.24410
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3ryc
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BUSTER
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	3.790
High resolution limit [Å]	3.570	3.570
Number of reflections	25304	3723
<I/σ(I)>	4.15	0.92
Completeness [%]	98.7
Redundancy	11.7
CC(1/2)	0.997	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG1500, LISO4, PIPES BUFFER, PH 6.80