6TIT

VSV G_440

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-10-15
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.987
Spacegroup name	P 6 2 2
Unit cell lengths	120.950, 120.950, 200.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.820 - 2.070
R-factor	0.2083
Rwork	0.207
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i2s
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.820	2.350
High resolution limit [Å]	2.070	2.070
Number of reflections	52870	6568
<I/σ(I)>	8.27
Completeness [%]	99.5
Redundancy	6.8
CC(1/2)	0.900	0.396

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	8% PEG8000, 0.15M calcium acetate, 0.15M HEPES pH7.5