6TIS
DROSOPHILA GDP-TUBULIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.969998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.588, 126.515, 250.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.300 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21850 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ryc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.180 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 93638 | 4226 |
| <I/σ(I)> | 10.3 | 1 |
| Completeness [%] | 98.8 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.999 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG1500, LISO4, PIPES BUFFER, PH 6.80 |
