6TIP
Engineered streptavidin variant (YNAFM) in complex with the Strep-tag II peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91801 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 57.560, 57.560, 181.929 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.630 - 2.100 |
R-factor | 0.2009 |
Rwork | 0.200 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qbb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.631 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 91.066 | 57.560 | 2.210 |
High resolution limit [Å] | 1.897 | 6.640 | 2.100 |
Rmerge | 0.217 | 0.338 | |
Rmeas | 0.165 | 0.234 | 0.361 |
Rpim | 0.054 | 0.084 | 0.123 |
Total number of observations | 173120 | 5928 | 22720 |
Number of reflections | 18752 | 700 | 2674 |
<I/σ(I)> | 8 | 10.6 | 4.5 |
Completeness [%] | 99.8 | 98.6 | 99.9 |
Redundancy | 9.2 | 8.5 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | ammonium sulfate, sodium acetate |