6THB
Receptor binding domain of the Cedar Virus attachment glycoprotein (G)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 201.460, 201.460, 112.890 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.241 - 2.780 |
R-factor | 0.2025 |
Rwork | 0.201 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vwd |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3228) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.000 | 2.850 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.164 | 1.776 |
Rpim | 0.037 | 0.412 |
Number of reflections | 65603 | 4849 |
<I/σ(I)> | 15.4 | 2 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 20.5 | 19.5 |
CC(1/2) | 0.998 | 0.777 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5% (w/v) polyethylene glycol 6000, 0.1 M MES pH 6.0 |