6TH0

Crystal structure of Arabidopsis thaliana NAA60 in complex with acetyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999998
Spacegroup name	P 21 21 21
Unit cell lengths	69.147, 71.997, 77.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.010 - 1.750
R-factor	0.2033
Rwork	0.202
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5icv
RMSD bond length	0.008
RMSD bond angle	0.981
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.010	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.059	2.036
Rpim	0.017	0.581
Number of reflections	39965	2180
<I/σ(I)>	16.9	1.1
Completeness [%]	100.0	100
Redundancy	12.8	13.1
CC(1/2)	0.999	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	reservoir: 0.1 M HEPES pH 7.5, 100 mM NaCl and 1.1 M NH4SO4 protein sample: c = 5 mg/ml drop composition: volume: 250 nl protein solution + 250 nl precipitant