6TGX

Crystal structure of Arabidopsis thaliana NAA60 in complex with a bisubstrate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976251
Spacegroup name	P 21 21 21
Unit cell lengths	50.062, 76.165, 109.226
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.510 - 1.770
R-factor	0.1825
Rwork	0.181
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292105306
RMSD bond length	0.011
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.510	1.810
High resolution limit [Å]	1.770	1.770
Rmerge	0.085	2.414
Rpim	0.025	0.700
Number of reflections	41529	2329
<I/σ(I)>	14.9	1.1
Completeness [%]	99.8	100
Redundancy	12.5	12.7
CC(1/2)	0.999	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291.15	reservoir: 0.1 M citric acid pH 5 and 20 % (w/v) PEG 6000 protein: c=13mg/ml drop: 200nl protein solution + 200 nl precipitant