6TER
Crystal structure of a galactokinase from Bifidobacterium infantis in complex with Galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.320, 164.929, 113.630 |
Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
Resolution | 82.600 - 1.680 |
R-factor | 0.2278 |
Rwork | 0.224 |
R-free | 0.30800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6tep |
RMSD bond length | 0.012 |
RMSD bond angle | 1.853 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.600 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.108 | 0.553 |
Number of reflections | 205822 | 10162 |
<I/σ(I)> | 4.9 | |
Completeness [%] | 94.7 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, 0.15 - 0.25 M NaCl and 18 - 22 % (w/v) polyethylene glycol 6000) |